Using Affinity Capillary Electrophoresis To ldentify the Peptide in a Peptide Library that Binds Most Tightly to vancomycinr
نویسندگان
چکیده
This paper describes a procedure, based on competitive binding, for identifying tight-binding ligand(l)Ig a receptor in mixtures of equimolar ligand candidates using affinity capillary electrophoresis (ACE). Vancomycin and a small library of 32 peptides were used as a model syatem to illushate the procedureThis procedure should be applicable to peptide mixtures of greater complerity than the one used here, as well as to mi-tutes of nonpeptidic compounds. Limits and limitations to the procedure are described.
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